Abstract 1. Aim and purpose Multidrug resistance (MDR) in P. aeruginosa has appeared as an issue of great concern with the emergence of Metallo-β-lactamase (MBL)-producing isolates, which showed to be the primary resistance mechanism in carbapenem-resistant P. aeruginosa. The aim of the present study was detection of MBL-producing P. aeruginosa isolates from patients and evaluate